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Agilent Eclipse Spectrofluorimeter

Collect fluorescence, phosphorescence, chemi- or bioluminescence spectra between 200 and 900 nm with up to 1.5 nm resolution. Variable temperature and steady-state polarization experiments are possible because the Agilent Eclipse is equipped with a four-cuvette, Peltier-thermostated sample changer and computer-controlled sheet polarizers. The excitation source is a pulsed Xenon arc lamp so it is possible to measure the fluorescence or phosphorescence lifetimes of long-lived excited species.

First-time users please contact the hub manager to arrange for training on the use of this instrument. Qualified users can book instrument time online with the user name eclipse_UBC. On the day(s) of your experiment(s), you will need to bring with you the following supplies :

  • fluorescence cuvette(s).
  • cleared samples (centrifuged or filtered)
  • pipetters and pipet tips
  • Parafilm
  • cleaning solutions (water, detergents, acid, alcohol, etc.)
  • Kimwipes (preferably lint free)
  • USB thumb drive(s)
  • printer paper

The minimum sample volume required for measurements is 0.5 ml when using a conventional 3.5 ml fluorescence cuvette (e.g., Hellma 101-10-40 or Starna 3-G-10) . For even smaller samples, however, it may be necessary to use micro- or ultra-microcuvettes (e.g., Hellma 105-251-15-40 or Starna 16.12F-Q-1.5/Z15). Specify a window "centre height" of 15 mm when purchasing such a small-volume cuvette, and note that cuvette placement in the sample holder may be difficult with cuvettes such as Hellma 105-253-15-40..

For detailed theoretical explanations, practical considerations and application examples, consult :

  • Principles of fluorescence spectroscopy by Joseph R. Lakowicz (ebook available from the UBC library to UBC staff, students and Faculty).
  • Principles and applications of fluorescence spectroscopy by Jihad Rene Albani (ebook available from the UBC library to UBC staff, students and Faculty).
  • New trends in fluorescence spectroscopy: applications to chemical and life science Bernard Valeur and Jean-Claude Brochon (eds.) (Main library QD96.F56 N48 2001 c.1).
  • An introduction to fluorescence spectroscopy by Ashutosh Sharma and Stephen G. Schulman (Main library QD96.F56 S383 1999 c.1).

Other seful resources about fluorescence spectroscopy are available from the ISS and Molecular Probes websites.