Varian Eclipse Fluorescence Spectrophotometer

Collect fluorescence, phosphorescence, chemi- and bioluminescence spectra between 200 and 900 nm with up to 1.5 nm resolution. The minimum sample volume required is 0.5 ml in a conventional 1x1 cm² fluorescence cuvette. The instrument is also equipped with polarizers for steady-state polarization measurements and, because the excitation source is a pulsed Xenon arc lamp, the Eclipse can be used to measure phosphorescence and fluorescence lifetimes of long-lived fluorophores. For more detailed information, please visit the Varian website for this fluorometer.

First-time users should obtain the passwords from the hub manager to book instrument time and to access the spectroscopy and kinetics suite. Then, reserve instrument time online with the username eclipse_UBC.

On the day of your experiments, you may need the following supplies:

• cleared samples (centrifuged or filtered)
• fluorescence cuvette(s)
• pipetters and pipet tips
• Parafilm
• cleaning solutions (water, detergents, acid, alcohol, etc.)
• Kimwipes (preferably lint free)
• floppy disk(s) (formatted for a PC)
• printer paper

For detailed theoretical explanations, practical considerations, and application examples, consult:

• Principles of fluorescence spectroscopy by Joseph R. Lakowicz
• New trends in fluorescence spectroscopy: applications to chemical and life science Bernard Valeur and Jean-Claude Brochon (eds.) (Main library QD96.F56 N48 2001 c.1).
• An introduction to fluorescence spectroscopy by Ashutosh Sharma and Stephen G. Schulman (Main library QD96.F56 S383 1999 c.1).

Other useful information can be obtained at the Molecular Probes website.

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