ISS K2 Spectrofluorometer

The K2 from ISS is a multifrequency phase and modulation spectrofluorometer for the determination of fluorescence lifetimes and rotational correlation times of molecules (lifetimes ~ ns or greater). For detailed information about this fluorometer and useful resources, please visit the ISS_website. Although this instrument can also be used to measure steady-state fluorescence, excitation and polarization spectra, users are encouraged to use the Eclipse fluorimeter for this purpose instead.

First-time users please contact the hub manager to arrange for training on the use of this instrument. Qualified users can book instrument time online with the user name k2fl_UBC. On the day(s) of your experiment(s), you will need to bring with you the following supplies :

  • fluorescence cuvettes (e.g., Hellma 101-10-40 or Starna 3-G-10)
  • cleared samples (centrifuged or filtered)
  • a reference fluorophore or glycogen
  • pipetters and pipet tips
  • Parafilm
  • cleaning solutions (water, detergents, acid, alcohol, etc.)
  • Kimwipes (preferably lint free)
  • USB thumb drive(s)
  • printer paper

For detailed theoretical explanations, practical considerations, and application examples, consult:

  • Principles of fluorescence spectroscopy by Joseph R. Lakowicz (ebook available from the UBC library to UBC staff, students and Faculty).
  • Principles and applications of fluorescence spectroscopy by Jihad Rene Albani (ebook available from the UBC library to UBC staff, students and Faculty).
  • New trends in fluorescence spectroscopy: applications to chemical and life science Bernard Valeur and Jean-Claude Brochon (eds.) (Main library QD96.F56 N48 2001 c.1).
  • An introduction to fluorescence spectroscopy by Ashutosh Sharma and Stephen G. Schulman (Main library QD96.F56 S383 1999 c.1).

Also, explore the website of the Center for Fluorescence Spectroscopy at the University of Maryland for more resources and software code for the analysis of the data kinetics obtained with the K2 fluorometer.